XRCC5 Antikörper (AA 462-475)

Produktnummer ABIN337306

Dieser Ziege Polyklonal Antikörper detektiert spezifisch XRCC5 in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für XRCC5 Antikörper (AA 462-475) (ABIN337306)

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser XRCC5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-XRCC5 Antikörper

Bindungsspezifität: AA 462-475

Spezifität: Human XRCC5 / Ku80.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-SLAKKDEKTDTLED from an internal region of human XRCC5 / Ku80 (NP_066964.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (93%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu XRCC5

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Andere Bezeichnung: XRCC5 / Ku80

Hintergrund: Name/Gene ID: XRCC5

Synonyms: XRCC5, 86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, KARP-1, Ku autoantigen, 80  kDa, NFIV, KARP1, Ku86, KUB2, TLAA, Nuclear factor IV, KU80, Thyroid-lupus autoantigen

Gen-ID: 7520

NCBI Accession: NP_066964

UniProt: P13010

Pathways: DNA Reparatur