GAPDH Antikörper (AA 73-87)

Produktnummer ABIN337259

Der Kaninchen Polyklonal anti-GAPDH Antikörper wird verwendet zum Nachweis von GAPDH in Proben von Human und Affe. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für GAPDH Antikörper (AA 73-87) (ABIN337259)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: AA 73-87

Spezifität: Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein was used as the immunogen.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset (100%) Pig (93%) Mouse, Rat, Cat, Chicken, Armadillo, Xenopus (87%).

Aufreinigung: Protein G purified

Immunogen: Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset (100%), Pig (93%), Mouse, Rat, Cat, Chicken, Armadillo, Xenopus (87%).

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gen-ID: 2597

UniProt: P04406