MMP 9 Antikörper

Produktnummer ABIN337212

Der Kaninchen Polyklonal anti-MMP 9 Antikörper (ABIN337212) detektiert spezifisch MMP 9 in WB, IHC, IP, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für MMP 9 Antikörper (ABIN337212)

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MMP 9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MMP 9 Antikörper

Spezifität: Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.

Aufreinigung: Protein A purified

Immunogen: E. coli expressed active rat 92kD type IV collagenase (catalytic domain).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr (1:50), IHC-P (5 μg/mL), IP (1:250), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Andere Bezeichnung: MMP9 / Gelatinase B

Hintergrund: Name/Gene ID: MMP9
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP9, 92kd gelatinase, 92 kd type iv collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Matrix metalloproteinase-9, MMP-9, Type V collagenase, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2

Gen-ID: 4318

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events