GCLC Antikörper (AA 295-313)

Produktnummer ABIN337056

Der Kaninchen Polyklonal anti-GCLC Antikörper (ABIN337056) detektiert spezifisch GCLC in WB, IHC, ELISA, IP und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh) und Affe.

Kurzübersicht für GCLC Antikörper (AA 295-313) (ABIN337056)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GCLC Antikörper

Bindungsspezifität: AA 295-313

Spezifität: Recognizes the heavy subunit of rat GCS. Species cross-reactivity: human, monkey and mouse. Cross-reacts weakly with bovine.

Aufreinigung: Protein A purified

Immunogen: A synthetic peptide derived from corresponding to aa295-313 of rat GCLC (heavy subunit of GCS).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 2.5-5 μg/mL for 2hrs at RT. Immunoprecipitation: 10 μg/mg protein lysate. Denatured verified. Use Protein A. Positive control: Jurkat, MAD109, PC12 cells.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: GCLC

Hintergrund: Name/Gene ID: GCLC

Synonyms: GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC

Gen-ID: 2729

UniProt: P48506

Pathways: Cell RedoxHomeostasis