Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M) (AA 355-366) Antikörper

Produktnummer ABIN337024

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Eukaryotic Translation Initiation Factor 3, Subunit M in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Fledermaus, Affe und Schwein.

Kurzübersicht für Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M) (AA 355-366) Antikörper (ABIN337024)

Target: Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Fledermaus, Affe, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: AA 355-366

Spezifität: Human EIF3M / PCID1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%) Hamster, Opossum, Platypus, Xenopus (92%) Turkey, Chicken (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-NAWKQNLNKVKN from the C-terminus of human EIF3M / PCID1 (NP_006351.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%), Hamster, Opossum, Platypus, Xenopus (92%), Turkey, Chicken (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Antigendetails

Target: Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M)

Andere Bezeichnung: EIF3M / PCID1

Hintergrund: Name/Gene ID: EIF3M

Synonyms: EIF3M, B5, B5 receptor, Dendritic cell protein, GA17, HFLB5, Fetal lung protein B5, Hfl-B5, TANGO7, PCID1

Gen-ID: 10480

NCBI Accession: NP_006351

UniProt: Q7L2H7

Pathways: Ribonucleoprotein Complex Subunit Organization