IFT74 Antikörper (AA 588-600)

Produktnummer ABIN337022

Der Ziege Polyklonal anti-IFT74 Antikörper wird verwendet zum Nachweis von IFT74 in Proben von Human und Affe. Er wurde validiert für ELISA, WB, IHC und IHC (p).

Kurzübersicht für IFT74 Antikörper (AA 588-600) (ABIN337022)

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IFT74 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IFT74 Antikörper

Bindungsspezifität: AA 588-600

Spezifität: Human IFT74.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%) Gibbon (92%) Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KTIVDALHSTSGN from the C-terminus of human IFT74 (NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%), Gibbon (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Andere Bezeichnung: IFT74 / CCDC2

Hintergrund: Name/Gene ID: IFT74

Synonyms: IFT74, CCDC2, CMG-1, CMG1

Gen-ID: 80173

NCBI Accession: NP_001092692, NP_001092693, NP_079379

UniProt: Q96LB3

Pathways: Hedgehog Signalweg, Chromatin Binding