BrdU Antikörper
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| Klonalität |
Monoklonal (BrdU 206) |
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Wirt
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Reaktivität
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Compound (28), Human (17), Maus (12), Ratte (Rattus) (12), Hund (5), Alle Spezies (4), Kaninchen (4), Schaf (4), Diverse Spezies (4), Huhn (3), Rind (Kuh) (3), Schwein (3), Pferd (1)
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Applikation
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 Alternativen Immunhistochemie (IHC), Immunhistochemie (Paraffinschnitte) (IHC (p))
Durchflusszytometrie (FACS) (55), Immunfluoreszenz (IF) (41), Immunhistochemie (IHC) (14), Immunhistochemie (Paraffinschnitte) (IHC (p)) (13), Intrazelluläre Durchflusszytometrie (ICFC) (8), ELISA (6), Western Blot (WB) (6), Immunpräzipitation (IP) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Immunzytochemie (ICC) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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| Produktnummer |
ABIN336860 |
| Menge |
7 ml (Varianten) |
| Preis |
140,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Applikationshinweise
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IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. (For staining of FFPE tissue sections, incubate with 4 N HCl for 20 minutes at RT, followed by treatment with proteolytic digestion for 10 minutes at RT. (Please refer to our Trypsin (AR-6541), Pronase (AR-6542) or pepsin (AR-6543) data sheets. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Liver of experimental animal injected with BrdU or cell line grown in presence of BrdU. Cellular Localization: Nuclear.
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Buffer
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The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a syn
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Lagerung
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2-8°C
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "BrdU", Typ "Antikörper" finden
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Wirte
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Kaninchen (41), Maus (21), Ratte (6), Schaf (2)
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Reaktivitäten
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Compound (28), Human (17), Maus (12), Ratte (Rattus) (12), Hund (5), Alle Spezies (4), Kaninchen (4), Schaf (4), Diverse Spezies (4), Huhn (3), Rind (Kuh) (3), Schwein (3), Pferd (1)
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Applikationen
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Durchflusszytometrie (FACS) (55), Immunfluoreszenz (IF) (41), Immunhistochemie (IHC) (14), Immunhistochemie (Paraffinschnitte) (IHC (p)) (13), Intrazelluläre Durchflusszytometrie (ICFC) (8), ELISA (6), Western Blot (WB) (6), Immunpräzipitation (IP) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Immunzytochemie (ICC) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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Konjugate
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FITC (7), Biotin (6), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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