E-cadherin Antikörper

Produktnummer ABIN335401

Dieser Maus Monoklonal Antikörper detektiert spezifisch E-cadherin in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für E-cadherin Antikörper (ABIN335401)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E-cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: SEC21

Produktdetails anti-E-cadherin Antikörper

Spezifität: Human.

Aufreinigung: Purified

Immunogen: SEC21 is a mouse monoclonal IgG1 antibody obtained by fusion of NS0 mouse myeloma cells with spleen cells from a mouse immunized with the synthetic peptide corresponding to amino acids 30-43 of mature human E-cadherin coupled to keyhole limpet hemocyanin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: SEC21 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part. The epitope is present within amino acid residues 30-43 of mature human E-cadherin. SEC21 is suitable for immunoblotting. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:1000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Andere Bezeichnung: E-Cadherin / Cadherin-1

Hintergrund: Cadherins constitute a family of transmembrane glycoproteins involved in Ca 2+ -dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin alpha E beta 7 . The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

Pathways: WNT Signalweg, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation