Lamin A/C (LMNA) Antikörper

Antigen: Lamin A/C (LMNA)

Synonyme: FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, CMT2B1, LGMD1B, Dhe, 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam, cb948, fk66d12, wu:fk66d12, lmna-A, MGC127505, LMNA, fpl, idc, lfp, cddc, emd2, fpld, hgps, ldp1, lmn1, lmnc, pro1, cdcd1, cmd1a, cmt2b1, lgmd1b, Lamin-A

Klonalität: Monoklonal (133A2)

Wirt: Maus

Reaktivität: Human, Ratte (Rattus), Maus, Rind (Kuh), Hund

Applikation: Durchflusszytometrie (FACS), Immunzytochemie (ICC), Immunhistochemie (IHC), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Western Blot (WB)

Produktnummer: ABIN335389

Produktbeschreibung

Weitere Bezeichnung: Lamin A

Immunogen: 133A2 is a mouse monoclonal IgG3/kappa antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.

Format: Purified

Isotyp: IgG3  (Passende Sekundärantikörper)

Klon: 133A2

Beschreibung: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Spezifität: Human, rat, mouse, bovine, dog.

Synonyme: FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, CMT2B1, LGMD1B, Dhe, 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam, cb948, fk66d12, wu:fk66d12, lmna-A, MGC127505, LMNA, fpl, idc, lfp, cddc, emd2, fpld, hgps, ldp1, lmn1, lmnc, pro1, cdcd1, cmd1a, cmt2b1, lgmd1b, Lamin-A

Anwendungen

Applikationshinweise: 133A2 recognizes an epitope located between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A. 133A2 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Lagerung: Store at 4 o C, or in small aliquots at -20 o C.

Forschungsgebiet: Signaltransduktion, Zytoskelett, Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Lamin A/C (LMNA) antibody

Publikationen

Publikationen: Hozuak, Sasseville, Raymond et al.: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995 (PubMed).

Machiels, Broers, Raymond et al.: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996 (PubMed).

Broers, Machiels, Kuijpers et al.: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997 (PubMed).

Machiels, Ramaekers, Kuijpers et al.: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997 (PubMed).

Jansen, Machiels, Hopman et al.: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997 (PubMed).

Pugh, Coates, Lane et al.: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997 (PubMed).

Neri, Raymond, Giordano et al.: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999 (PubMed).