VTCN1 Antikörper (Internal Region)

Produktnummer ABIN334524

Der Ziege Polyklonal anti-VTCN1 Antikörper (ABIN334524) detektiert spezifisch VTCN1 in WB, FACS, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für VTCN1 Antikörper (Internal Region) (ABIN334524)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VTCN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-VTCN1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: B7-H4

Sequenz: SKGKGNANLE YK

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SKGKGNANLEYK, from the internal region of the protein sequence according to NP_078902.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 26+21 kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26 kDa and 21 kDa band observed in Human Adrenal Gland lysates, and approx 27 kDa in Human Pancrea

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recomme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu VTCN1

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Andere Bezeichnung: VTCN1

Hintergrund: B7-H4, B7H4, B7S1, B7X, B7h.5, FLJ22418, PRO1291, RP11-229A19.4, VCTN1, T cell costimulatory molecule B7x, immune costimulatory protein, V-set domain containing T cell activation inhibitor 1

Molekulargewicht: 30.9kDa

Gen-ID: 79679, 242122, 295322

NCBI Accession: NP_078902, NP_001240778