NR1H4 Antikörper (Internal Region)

Produktnummer ABIN334396

Dieser Ziege Polyklonal Antikörper detektiert spezifisch NR1H4 in ELISA, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NR1H4 Antikörper (Internal Region) (ABIN334396)

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NR1H4 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-NR1H4 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Farnesoid X receptor / FXR

Sequenz: KSCREKTELT PDQQ

Kreuzreaktivität: Rind (Kuh), Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 55 kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5 μg/mL antibody staining (calculated MW of 54.4 kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NR1H4

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Andere Bezeichnung: NR1H4

Hintergrund: Farnesoid X receptor, HRR1, NR1H4, nuclear receptor subfamily 1, group H, member 4 , BAR, FXR, HRR-1, RIP14 , farnesol receptor HRR-1

Gen-ID: 9971

NCBI Accession: NP_005114

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process