NR4A1 Antikörper

Produktnummer ABIN317895

Der Kaninchen Polyklonal anti-NR4A1 Antikörper (ABIN317895) detektiert spezifisch NR4A1 in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für NR4A1 Antikörper (ABIN317895)

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NR4A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NR4A1 Antikörper

Spezifität: This antibody detects endogenous levels of Nur77/NR4A1 protein. (region surrounding Gly347)

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Andere Bezeichnung: NAK1

Hintergrund: Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate.Synonyms: Early Response Protein NAK1, GFRP1, HMR, N10, NR4A1, NUR77, Orphan Nuclear Receptor HMR, ST-59, TR3 Orphan Receptor

Molekulargewicht: approx. 60 kDa

Gen-ID: 3164

NCBI Accession: NP_002126

UniProt: P22736

Pathways: Fc-epsilon Rezeptor Signalübertragung, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Steroid Hormone Mediated Signaling Pathway