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6x-HIS Tag Antikörper

Antigen

6x-HIS Tag

Klonalität Monoklonal (IPA2C5-1)
Wirt
Applikation
Alternativen Western Blot (WB)
Produktnummer ABIN306800
Menge 100 ug  (1 mg/ml)
Preis 244,10 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferbar innerhalb von 5 bis 10 Werktagen

Produktbeschreibung

Isotyp IgG2a  (Passende Sekundärantikörper)
Klon IPA2C5-1
Beschreibung 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only. Synonyms: Anti-6x-Histidine Tag Antibody.

Anwendungen

Applikationshinweise Detects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines.
Konzentration 1 mg/ml
Lagerung Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "6x-HIS Tag", Typ "Antikörper" finden
Wirte Ziege (5), Kaninchen (3), Maus (1)
Applikationen ELISA (7), Immunfluoreszenz (IF) (7), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (7), Western Blot (WB) (7), Immunpräzipitation (IP) (1)
Konjugate HRP (2), Agarose (1), FITC (1)