Phosphotyrosine Antikörper (Peroxidase (POD))

Produktnummer ABIN306704

Dieser Maus Monoklonal Antikörper detektiert spezifisch Phosphotyrosine in WB, IP, IHC und EIA.

Kurzübersicht für Phosphotyrosine Antikörper (Peroxidase (POD)) (ABIN306704)

Target: Phosphotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist konjugiert mit Peroxidase (POD)

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Klon: PY20

Produktdetails anti-Phosphotyrosine Antikörper (Peroxidase (POD))

Verwendungszweck: Mouse anti Phosphotyrosine, conjugated with peroxidase

Produktmerkmale: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.

Aufreinigung: Prot A/G

Immunogen: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Application: A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 60 % glycerol, phosphate buffered saline, and 0.1 % 2-Chloroacetamide

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate