Heat Shock 105kDa/110kDa Protein 1 (HSPH1) Antikörper

Antigen: Heat Shock 105kDa/110kDa Protein 1 (HSPH1)

Synonyme: hsph1, hsp105, MGC75864, hsph1b, MGC80255

Klonalität: Monoklonal (J1G12)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN306454

Produktbeschreibung

Weitere Bezeichnung: HSP105 alpha

Immunogen: Recombinant human HSP105 alpha (1-858 aa), Accession / GI number: NP_006635

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: J1G12

Beschreibung: Hsp105 is a mammalian member of the HSP105/110 family, a diverged subgroup of the HSP70 family. HSP105 exists as two isoforms, alpha and beta. Hsp105 alpha associates with Hsp70/Hsc70 as complexes in vivo and regulates the chaperone activity of Hsp70/Hsc70 negatively in vitro and in vivo. Hsp105 a is constitutively expressed and also induced by various stress, whereas Hsp105 beta, lacking 44 amino acids from Hsp105 a, is specifically expressed during mild heat shock. Although Hsp105 a is shown to localize in the cytoplasm of mammalian cells, cellular localization of Hsp105 beta is not knownConcentration: 1 mg/ml

Anwendungen

Applikationshinweise: Recommended dilution 1:1000

Konzentration: 1mg/ml

Reinigung: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography

Buffer: In Phosphate-Buffered Saline (pH7.4) with 0.1% Sodium Azide

Lagerung: Store at -20°, Aliquot to avoid repeated freezing and thawing.

Forschungsgebiet: Hitzeschockproteine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis: Cell lysates of HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSP105 ? (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.