POLK Antikörper (AA 817-830)

Produktnummer ABIN303130

Dieser Ziege Polyklonal Antikörper detektiert spezifisch POLK in WB, IF, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für POLK Antikörper (AA 817-830) (ABIN303130)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POLK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-POLK Antikörper

Bindungsspezifität: AA 817-830

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunofluorescence. Western Blot (1/500-1/3000). ELISA (1/2000-1/10000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.8 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Details zu POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Andere Bezeichnung: DNA Polymerase kappa

Hintergrund: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.Synonyms: DINB protein, DINB1, DINP, POLK

Gen-ID: 51426

NCBI Accession: NP_057302

UniProt: Q9UBT6

Pathways: DNA Reparatur