RPA3 Antikörper

Produktnummer ABIN302303

Der Maus Monoklonal anti-RPA3 Antikörper wird verwendet zum Nachweis von RPA3 in Proben von Human und Maus. Er wurde validiert für WB, IP und IHC (p).

Kurzübersicht für RPA3 Antikörper (ABIN302303)

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RPA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 11-01-14

Produktdetails anti-RPA3 Antikörper

Spezifität: This antibody detects Replication Protein A3 (14 kDa) (RPA3).

Produktmerkmale: Synonyms: REPA3, RP-A p14, RFA3, RF-A protein 3, Replication protein A, Replication protein A 14 kDasubunit, Replication factor A protein 3

Aufreinigung: Protein G column

Immunogen: Full-length recombinant human RPA-14 expressed in E. coli

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Phosphate buffered saline

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu RPA3

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Hintergrund: Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.Synonyms: REPA3, RF-A protein 3, RFA3, RP-A p14, Replication factor A protein 3, Replication protein A, Replication protein A 14 kDa subunit

Gen-ID: 6119

UniProt: P35244

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA