GCPII / PSMA Antikörper

Antigen: GCPII / PSMA

Klonalität: Monoklonal (GCP-05)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunzytochemie (ICC)

Produktnummer: ABIN302088

Produktbeschreibung

Immunogen: amino acids 44-750 of human GCPII

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: GCP-05

Beschreibung: Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidicdipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membraneantigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoproteinexpressed in various tissues. In nervous system GCPII cleaves abundantN-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner,to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic,GCPII contributes to pathological conditions regarding e.g. Alzheimer's disease,Huntington's disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to bean interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamateto folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII,which is present in a number of tissues at low levels, is overexpressed in neovasculature ofmost solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer.Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated atthe N-terminus (PSM') than mRNA for membrane-bound GCPII, and this ratio is reversedupon malignant transformation.

Spezifität: The antibody GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II(NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoproteinexpressed mainly in tumour neovasculatures, nervous system and jejunum, which is animportant prostate tumour marker.

Anwendungen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow cytometry analysis of GCPII (PSMA) by various monoclonal antibodies (GCP-01, GCP-02, GCP-04, GCP-05) on LNCaP cell line.Legend:green column - 1 ug/ml, LNCaP cell lineblue column - 5 ug/ml, LNCaP cell lineyellow column - negative cell line