CD24 Molecule (CD24) Antikörper

Antigen: CD24 Molecule (CD24)

Synonyme: HSA, Cd24, Ly-52, nectadrin, CD24A, FLJ22950, FLJ43543, MGC75043

Klonalität: Monoklonal (SN3)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN302076

Produktbeschreibung

Weitere Bezeichnung: CD24

Immunogen: Glycoproteins purified from human NALM-1 cell line.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: SN3

Beschreibung: CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-likeGPI-anchored extracellular membrane glycoprotein expressed on several cell types,including B cells. When B cells are activated and induced to further maturation, however,CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, therebyregulating the activity of integrins and other proteins such as the chemokine receptorCXCR4, it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cellprecursors but not in mature resting B cells, where it instead inhibits their ability to proliferatein response to activation. CD24 expression is associated with invasiveness and poorerprognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Spezifität: The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen.CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cellmalignancies. It is not expressed on human thymocytes.HLDA IV, WS Code B 136HLDA V, WS Code B CD24.7

Anwendungen

Applikationshinweise: Immunohistochemistry (frozen sections) - Positive tissue: tonsil

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-G affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of human peripheral blood cells with anti-CD24 (SN3) Alexa Fluor® 700.