Linker For Activation of T Cells (LAT) Antikörper

Antigen: Linker For Activation of T Cells (LAT)

Synonyme: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Klonalität: Monoklonal (LAT-01)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN302069

Produktbeschreibung

Weitere Bezeichnung: LAT

Immunogen: Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmicdomain of human LAT.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: LAT-01

Beschreibung: LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembraneadaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Afterimmunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, orTec-family kinases, providing docking sites for downstream signaling molecules. LAT isessential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as wellas for maturation of early thymocytes. It is also involved in NK cell signaling and plateletactivation.

Spezifität: The antibody LAT-01 reacts with Linker for activation of T cells (LAT), a 36-38 kDatransmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells andplatelets. This antibody does not react with: Mouse, Other not tested.

Anwendungen

Applikationshinweise: Western BlottingImmunoprecipitation. Application note: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow Cytometry

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Signaltransduktion, Adaptive Immunität

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow Cytometry analysis