GRP78 Antikörper (AA 550-600)

Produktnummer ABIN298116

Der Kaninchen Polyklonal anti-GRP78 Antikörper (ABIN298116) detektiert spezifisch GRP78 in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GRP78 Antikörper (AA 550-600) (ABIN298116)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GRP78 Antikörper

Bindungsspezifität: AA 550-600

Spezifität: A portion of amino acids 550-600 of human BiP was used as the immunogen.

Aufreinigung: Protein G purified

Immunogen: A portion of amino acids 550-600 of human GRP78.

Type of Immunogen: Protein fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: HSPA5 / GRP78 / BIP

Hintergrund: Name/Gene ID: HSPA5

Synonyms: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78

Gen-ID: 3309

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling