DKK1 Antikörper (AA 250-262)

Produktnummer ABIN296913

Der Ziege Polyklonal anti-DKK1 Antikörper (ABIN296913) detektiert spezifisch DKK1 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Hund, Rind (Kuh), Pferd, Ziege, Schwein und Affe.

Kurzübersicht für DKK1 Antikörper (AA 250-262) (ABIN296913)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reaktivität: Human, Maus, Hund, Rind (Kuh), Pferd, Ziege, Schwein, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DKK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DKK1 Antikörper

Bindungsspezifität: AA 250-262

Spezifität: Human DKK1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%) Gibbon, Rat, Bat, Hamster, Platypus (92%) Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DHHQASNSSRLHT from the C-terminus of human DKK1 (NP_036374.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%), Gibbon, Rat, Bat, Hamster, Platypus (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Andere Bezeichnung: DKK1

Hintergrund: Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK

Gen-ID: 22943

NCBI Accession: NP_036374

UniProt: O94907

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation