HTRA3 Antikörper (AA 250-300)

Produktnummer ABIN296848

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HTRA3 in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Affe.

Kurzübersicht für HTRA3 Antikörper (AA 250-300) (ABIN296848)

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Reaktivität: Human, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HTRA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HTRA3 Antikörper

Bindungsspezifität: AA 250-300

Spezifität: The amino acid sequence is 100 % homologous in HtrA3, 80 % homologous in HtrA1 and 71 % homologous in HtrA2 and HtrA4.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%) Gorilla, Bovine, Pig, Pufferfish (93%) Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%) Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).

Aufreinigung: Protein G purified

Immunogen: Amino acids 273-287 (IVSTAQREGRELGLR)of human HtrA3 were used as immunogen. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%), Gorilla, Bovine, Pig, Pufferfish (93%), Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%), Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu HTRA3

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Andere Bezeichnung: HTRA3

Hintergrund: Name/Gene ID: HTRA3
Subfamily: Serine S1C
Family: Protease

Synonyms: HTRA3, HtrA serine peptidase 3, Serine protease HTRA3, Tasp, Probable serine protease HTRA3, PRSP

Gen-ID: 94031

UniProt: P83110