Collagen Type I Antikörper (Biotin)

Produktnummer ABIN2619406

Der Biotin-konjugierte Kaninchen Polyklonal anti-Collagen Type I Antikörper (ABIN2619406) detektiert spezifisch Collagen Type I in WB, IHC, ELISA, IP und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für Collagen Type I Antikörper (Biotin) (ABIN2619406)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Collagen Type I Antikörper (Biotin)

Spezifität: Collagen Type I from adult human knee cartilage and bovine nasal cartilage.

Aufreinigung: Affinity purified

Immunogen:

Type of Immunogen: Native protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:3000 - 1:6000), IHC, IHC-P (10 μg/mL), IP, WB (1:3000 - 1:6000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: 0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA,  pH 8.0, 10 mg/mL BSA, 0.01 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen I