UCP2 Antikörper

Produktnummer ABIN289564

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch UCP2 in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für UCP2 Antikörper (ABIN289564)

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UCP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-UCP2 Antikörper

Spezifität: This antibody reacts with residues 125-142 EETAKKVRRAIEQLAAMD of the human Survivin protein. Cross reacts with mouse and rat Survivin (88% sequenceidentity).

Kreuzreaktivität (Details): This UCP2 peptide sequence is 100 % conserved in rat, hamster, and 86 % in human, pig, canine, and bovine UCP2. It has no significant homology with UCP1, UCP3-5.

Aufreinigung: Affinity chromatography purified

Immunogen: UCP2 antibody was raised in rabbit using a 14 amino acid peptide from mouse UCP2 as the immunogen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in PBS to a concentraiton of 1 mg/mL.

Konzentration: Lot specific

Buffer: Affinity purified antibody lyophilized from PBS with 0.1 % BSA.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Details zu UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Andere Bezeichnung: UCP2

Hintergrund: Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and it is only 275 aa long. Mouse/rat UCP2 is A 309 AA (human 309 aa chromosome 7, ~95% homology) mitochondrial uncoupling protein. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation.

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport