The peptide sequence used to generate this antibody has no significant homology with UCP2 or UCP1.0.2% BSA & 0.05% NaN3
Kreuzreaktivität (Details)
This human UCP3 peptide sequences is 100 % conserved in long and short form of human UCP3, 88 % in monkey, 78 % in humming bird, 76 % in canine and pig, 70 % in mouse, bovine, hamster, and 64 % in rat UCP3. UCP31-P has no significant homology with UCP1-2 or UCP4-5.
Aufreinigung
Affinity chromatography purified
Immunogen
UCP3 antibody was raised in rabbit using a 17 amino acid peptide sequence located at the 2 nd and 2 d transmembrane domain of human UCP3 as the immunogen.
UCP3
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with sterile deionised water.
Konzentration
Lot specific
Buffer
Lyophilized from PBS with 0.1 % BSA, and 0.,02 % merthiolate.
Handhabung
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Target
UCP3
(Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))
Andere Bezeichnung
UCP3
Hintergrund
Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and it is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.