Phosphotyrosine Antikörper

Produktnummer ABIN285915

Der Kaninchen Polyklonal anti-Phosphotyrosine Antikörper (ABIN285915) detektiert spezifisch Phosphotyrosine in WB, IP, IHC und ELISA.

Kurzübersicht für Phosphotyrosine Antikörper (ABIN285915)

Target: Phosphotyrosine

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA

Produktdetails anti-Phosphotyrosine Antikörper

Kreuzreaktivität (Details): Recognize proteins and peptides phosphorylated on tyrosine residues. Does not cross - react with phosphoserine and threonine.

Aufreinigung: Affinity purified

Immunogen: Phosphotyrosine antibody was raised in rabbit using Iodoacetylphosphotyrosine-KLH conjugate as the immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1:1,000, IHC: 1:50, IP: 10 µg/500 µg protein sample, WB: 1:250
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: Tris-acetate buffer, pH 7-7.5, with 0.05 % NaN3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phosphotyrosine which is considered as a key step in the regulation of enzymatic activities such as signal transduction.