Protein SET (SET) Antikörper

Antigen: Protein SET (SET)

Synonyme: 2PP2A, IGAAD, TAF-I, I2PP2A, IPP2A2, PHAPII, TAF-IBETA, AA407739, StF-IT-1, MGC118419, 2610030F17Rik, 5730420M11Rik, Ab1-115

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN265241

Produktbeschreibung

Produktmerkmale: Synonyms: I-2PP2A, TAF-I, PHAPII, IGAAD, Phosphatase 2A inhibitor I2PP2A, Template-activating factorI, Inhibitor of granzyme A-activated DNase

Weitere Bezeichnung: SET / I2PP2A

Swiss-Prot: Q01105

Immunogen: Synthetic peptide derived from the human SET protein

Kreuzreaktivität: Human

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Human SET was originally identified as part of the SET-CAN fusion gene produced by asomatic translocation event in a patient with acute undifferentiated leukemia. Indeveloping kidney, SET is highly expressed in the zone of nephron morphogenesis. SEThas been shown to be a potent and specific inhibitor of protein phosphatase 2A, a familyof major serine/threonine phosphatases involved in regulating cell proliferation anddifferentiation. SET is also involved in the regulation of renal cell proliferation andtumorigenesis. SET mRNA expression is markedly reduced in cells rendered quiescent byserum starvation, contact inhibition, or differentiation. SET protein expression is also muchgreater in developing rat and human kidney than in fully differentiated, mature kidney. High levels of SET mRNA and SET protein expression arefound in Wilms' tumor, but not inrenal cell carcinoma, adult polycystic kidney disease or in transitional cell carcinoma.

Spezifität: This antibody detects I2PP2A / SET, widely expressed. Low levels in quiescent cells duringserum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Western blot (1: 400 dilution). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: Lot specific

Reinigung: Aff - Purified

Buffer: Phosphate buffered saline with 0.08% sodium azide

Lagerung: Store the product (in aliquots) at -20 °C to -70 °C. Can be shipped at 2 - 8 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: von Lindern, van Baal, Wiegant et al.: "Can, a putative oncogene associated with myeloid leukemogenesis, may be activated by fusion of its 3' half to different genes: characterization of the set gene." in: Molecular and cellular biology, Vol. 12, Issue 8, pp. 3346-55, 1992 (PubMed).

Nagata, Kawase, Handa et al.: "Replication factor encoded by a putative oncogene, set, associated with myeloid leukemogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 10, pp. 4279-83, 1995 (PubMed).

Li, Makkinje, Damuni: "Molecular identification of I1PP2A, a novel potent heat-stable inhibitor protein of protein phosphatase 2A." in: Biochemistry, Vol. 35, Issue 22, pp. 6998-7002, 1996 (PubMed).

Seo, McNamara, Heo et al.: "Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein." in: Cell, Vol. 104, Issue 1, pp. 119-30, 2001 (PubMed).

Minakuchi, Kakazu, Gorrin-Rivas et al.: "Identification and characterization of SEB, a novel protein that binds to the acute undifferentiated leukemia-associated protein SET." in: European journal of biochemistry / FEBS, Vol. 268, Issue 5, pp. 1340-51, 2001 (PubMed).

Fan, Beresford, Oh et al.: "Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor." in: Cell, Vol. 112, Issue 5, pp. 659-72, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Tsujio, Zaidi, Xu et al.: "Inhibitors of protein phosphatase-2A from human brain structures, immunocytological localization and activities towards dephosphorylation of the Alzheimer type hyperphosphorylated tau." in: FEBS letters, Vol. 579, Issue 2, pp. 363-72, 2005 (PubMed).

Kim, Sprung, Chen et al.: "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." in: Molecular cell, Vol. 23, Issue 4, pp. 607-18, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Molina, Horn, Tang et al.: "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2199-204, 2007 (PubMed).

Shih, Shih, Lee et al.: "Particle size distributions and health-related exposures of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) of sinter plant workers." in: Chemosphere, 2009 (PubMed).