His Tag Antikörper

Produktnummer ABIN264410

Der Maus Monoklonal anti-His Tag Antikörper wird verwendet zum Nachweis von His Tag. Er wurde validiert für WB.

Kurzübersicht für His Tag Antikörper (ABIN264410)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: IPA2C5-1

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Produktmerkmale: Synonyms: 6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag

Aufreinigung: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized withHis-His-His-His-His-His synthetic peptide and mouse myeloma cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.