Heat Shock Protein 65 (HSP65) Antikörper

Antigen: Heat Shock Protein 65 (HSP65)

Synonyme: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60

Klonalität: Monoklonal (BDI578)

Wirt: Maus

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN263881

Produktbeschreibung

Produktmerkmale: Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04

Weitere Bezeichnung: Heat shock protein 65 / HSP65

Swiss-Prot: P0A520

Immunogen: Purified protein derivative (PPD)

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: BDI578

Beschreibung: Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infectionbegins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism isdetermined by its ability to escape host immune responses as well as eliciting delayedhypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mountan effective defense. Several virulence factors are responsible for this apparent failure,most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, andthe 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created newinterest in clinical diagnosis. Studies have shown immunohistochemical techniques to besuperior to conventional special stains. Thus the demonstration of mycobacterial antigensare not only useful in establishing mycobacterial aetiology, but can also be used as analternative method to the conventional Ziehl-Neelsen method.

Spezifität: This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp. , B. subtilis, S. pneumoniae,and E. coli.

Anwendungen

Applikationshinweise: ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0,1 mg/ml (OD280nm, E0.1% = 1.4)

Reinigung: Purified

Buffer: 0.01M PBS, pH 7.2 containing 0,09% sodium azide

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar