G Protein-Coupled Receptor 125 (GPR125) Antikörper

Antigen: G Protein-Coupled Receptor 125 (GPR125)

Synonyme: AU044632, 3830613O22Rik, PGR21, TEM5L

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN263209

Produktbeschreibung

Weitere Bezeichnung: GPR125

Gen-ID: 166647

Immunogen: CTNKSASSFKNS

Format: Affinity-purified

Beschreibung: All names and symbols: GPR125 , G protein-coupled receptor 125 , PGR21, TEM5L, TEM5-like. Official Symbol: GPR125 . RefSeq number(s): NP_660333.2

Spezifität: Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN263209 (0.5µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.