TRPV5 Antikörper (Internal Region)

Produktnummer ABIN263199

Zitiert in 1 Publikation. Der Ziege Polyklonal anti-TRPV5 Antikörper (ABIN263199) detektiert spezifisch TRPV5 in WB, ELISA, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TRPV5 Antikörper (Internal Region) (ABIN263199)

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRPV5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Güteklasse: Verified

Produktdetails anti-TRPV5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TRPV5

Sequenz: SHRGWEILRQ NT

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SHRGWEILRQNT, from the internal region of the protein sequence according to NP_062815.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 85 kDa band observed in Human Pancreas lysates (calculated MW of 82.6 kDa according to NP_062815.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Kennedy

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF on Human: Kennedy BG et al. (2010) PMID: 20405023. This antibody has been successfully used in ICC on Mouse: Mariana Labarca et al. (2015) PMID: 2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-TRPV5 Antikörper (ABIN263199)

Kennedy, Torabi, Kurzawa, Echtenkamp, Mangini: "Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium." in: Molecular vision, Vol. 16, pp. 665-75, (2010) (PubMed).

Details zu TRPV5

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Andere Bezeichnung: TRPV5

Hintergrund: TRPV5 , transient receptor potential cation channel, subfamily V, member 5 , CAT2, ECAC1, OTRPC3, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3

Gen-ID: 56302, 194352, 116469

NCBI Accession: NP_062815