ATP6AP1 Antikörper

Produktnummer ABIN249357

Zitiert in 8+ Publikationen. Der Kaninchen Polyklonal anti-ATP6AP1 Antikörper (ABIN249357) detektiert spezifisch ATP6AP1 in WB, IF und IL. Dieser Antikörper reagiert spezifisch mit Proben aus Soybean, Arabidopsis thaliana und Chlamydomonas.

Kurzübersicht für ATP6AP1 Antikörper (ABIN249357)

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Reaktivität: Soybean, Arabidopsis thaliana, Chlamydomonas

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6AP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunolocalization (IL)

Produktdetails anti-ATP6AP1 Antikörper

Kreuzreaktivität: Sojabohne (Glycine max)

Kreuzreaktivität (Details): No cross-reactivity with: Aspergillus niger

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 95 kDa (Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 1000 - 1 : 5000 with standard ECL OR 1 : 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1 : 600 - 1 : 1000 (IF).
Cellular [compartment marker] for plasma membrane

Kommentare:

for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Do not store this antibody at 4 °C.

Referenzen für anti-ATP6AP1 Antikörper (ABIN249357)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Chen, Fujii, Yamaji, Masuda, Takemoto, Kamiya, Yusuyin, Iwasaki, Kato, Maeshima, Ma, Ueno: "Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family." in: Journal of experimental botany, Vol. 64, Issue 14, pp. 4375-87, (2013) (PubMed).

Menckhoff, Mielke-Ehret, Buck, Vuletić, Lüthje: "Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: Native versus recombinant protein." in: Journal of proteomics, Vol. 80C, pp. 66-77, (2013) (PubMed).

Visnovitz, Solti, Csikós, Fricke: "Plasma membrane H(+) -ATPase gene expression, protein level and activity in growing and non-growing regions of barley (Hordeum vulgare) leaves." in: Physiologia plantarum, Vol. 144, Issue 4, pp. 382-93, (2012) (PubMed).

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Chen, Xiao, Wu, Dong, He, Pei, Zheng: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." in: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, (2010) (PubMed).

Page, Kropat, Hamel, Merchant: "Two Chlamydomonas CTR copper transporters with a novel cys-met motif are localized to the plasma membrane and function in copper assimilation." in: The Plant cell, Vol. 21, Issue 3, pp. 928-43, (2009) (PubMed).

Details zu ATP6AP1

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Andere Bezeichnung: H+ATPase

Hintergrund: AGI Code: At2g18960
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Molekulargewicht: 95 kDa (Arabidopsis thaliana)

UniProt: P20649

Pathways: Proton Transport, SARS-CoV-2 Protein Interaktom