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ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5) Antikörper

Antigen

ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5)

Synonyme 9230106F14Rik, RGD1566342, MDR1, wu:fi81f06, ABCB5beta, EST422562, ABCB5alpha, ABCB5, mdr1, im:7158730, wu:fc18f02
Klonalität Polyklonal
Wirt
Alternativen

Ziege

Reaktivität
Alternativen

Human

Applikation
Alternativen ELISA, Western Blot (WB), Durchflusszytometrie (FACS)
1 Publikation vorhanden
Produktnummer ABIN238622
Menge 100 µg  (100µg/200µl)
Preis 286,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung ABCB5
Gen-ID 340273
Swiss-Prot NP_848654.3
Immunogen Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3, NP_001157413.1.
Kreuzreaktivität Human
Format Liquid
Beschreibung This antibody is expected to recognise both reported isoforms (NP_848654.3, NP_001157413.1). Principal Names: ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5, EST422562, ABCB5beta, ABCB5alpha, ATP-binding cassette, sub-family B (MDR/TAP), member 5. Official Symbol: ABCB5
Spezifität Human
Molekulargewicht 89.8kDa

Anwendungen

Applikationshinweise Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 90kDa band observed in Human Skin lysates and in lysates of cell line A431 (calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1µg/ml. Flow cytometry: This antibody has been successfully used in FC for Hep3B cells as described in the following paper: Cheung et al, Gastroenterology. 2011 Jan,140(1):344-55, PMID: 20682318. Data obtained from a former batch.
Konzentration 100µg/200µl
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Lagerung Aliquot and store at -20°C. Minimize freezing and thawing.
Beschränkungen Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen Frank, Margaryan, Huang et al.: "ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma." in: Cancer research, Vol. 65, Issue 10, pp. 4320-33, 2005 (PubMed).

Alternativen

Alternativen zu Antigen "ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5)", Typ "Antikörper" finden
Wirte Kaninchen (28), Ziege (5)
Reaktivitäten Human (32), Maus (21), Ratte (Rattus) (21), Rind (Kuh) (18), Hund (18), Pferd (17), Kaninchen (3), Affe (2), Schwein (2), Schaf (1)
Applikationen Durchflusszytometrie (FACS) (20), Immunfluoreszenz (IF) (19), Western Blot (WB) (15), Enzyme Immunoassay (EIA) (8), ELISA (7), Immunhistochemie (Paraffinschnitte) (IHC (p)) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
Konjugate Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitope N-Term (3)