Transferrin Receptor (p90, CD71) (TFRC) Antikörper (APC)

Antigen: Transferrin Receptor (p90, CD71) (TFRC)

Synonyme: TFR, CD71, TFR1, TRFR, TR, p90, Trfr, Mtvr1, Mtvr-1, AI195355, AI426448, AU015758, 2610028K12Rik, E430033M20Rik, TFRC, tfr, cd71, tfr1, trfr, DKFZp469M0811, LOC100219280

Klonalität: Monoklonal (MEM-75)

Wirt: Maus

Reaktivität: Human

Konjugat: APC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN238474

Produktbeschreibung

Weitere Bezeichnung: CD71 / TFRC

Swiss-Prot: P02786

Immunogen: NALM-6 human pre-B cell line

Kreuzreaktivität: Human

Isotyp: IgG1

Klon: MEM-75

Beschreibung: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. Alternate Names: Transferrin Receptor.

Spezifität: The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.

Anwendungen

Applikationshinweise: Suitable for Flow Cytometry analysis of human blood cells using 10 m l reagent / 100 m l of whole blood or 10e6 cells in a suspension. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.

Lagerung: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar