Endoglin (ENG) Antikörper
| Antigen |
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| Synonyme |
END, ORW, HHT1, ORW1, CD105, FLJ41744, AI528660, AI662476, S-endoglin, Eng, MGC124909, ENG, MGC137842, DKFZp469D0419 |
| Klonalität |
Monoklonal (MEM-229) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)
Durchflusszytometrie (FACS) (123), Western Blot (WB) (70), Immunpräzipitation (IP) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (23), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (14), Enzyme Immunoassay (EIA) (11), ELISA (5), ELISA (Detektionsantikörper) (3), Immunfluoreszenz (IF) (2), Funktionale Studien (Func) (1), Immunzytochemie (ICC) (1)
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| Produktnummer |
ABIN238459 |
| Menge |
0.1 mg (1.0 mg/ml) |
| Preis |
190,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: ENG, END, HHT1, ORW, ORW1
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Weitere Bezeichnung
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CD105 / Endoglin
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Swiss-Prot
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P17813
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Immunogen
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Recombinant Vaccinia virus containing the Human CD105 (L-isoform) cDNA.
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Kreuzreaktivität
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Human, Schwein
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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MEM-229
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Beschreibung
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CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence ofTGFβR-2 as a receptor for TGFβ-1 and TGFβ-3. CD105 is highly expressed on endothelial cellsand promotes angiogenesis during wound healing, infarcts and in a wide range of tumoursand its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxicendothelial cells and also antagonises the inhibitory effects of TGFβ-1 on vascularendothelial cell growth and migration. Normal cellular levels of CD105 are required forformation of new blood vessels.
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Spezifität
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The antibody MEM-229 recognizes CD105 (Endoglin), a 180 kDa type I integral membranehomodimer glycoprotein expressed on vascular endothelial cells (small and large vessels),activated monocytes and tissue macrophages, stromal cells of certain tissues includingbone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal andadult bone marrow, it is also present on syncytiotrophoblast on placenta throughoutpregnancy. Species: Human, Porcine. Others not tested.
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Applikationshinweise
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Flow Cytometry: 10 μg/mlPositive Control: Kg1 Human acute myelogenous leukemia cell lineWestern Blotting (Non-Reducing Conditions). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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1.0 mg/ml
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Reinigung
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Purified
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Buffer
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PBS, pH~7.4 with 15 mM Sodium Azide as preservative.
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Lagerung
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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CD Antigene, Oberflächenrezeptoren der Immunzellen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Endoglin (ENG)", Typ "Antikörper" finden
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Wirte
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Maus (93), Ratte (31), Kaninchen (29), Huhn (1)
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Reaktivitäten
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Human (110), Maus (38), Schwein (13), Pferd (6), Ratte (Rattus) (3), Rind (Kuh) (1)
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Applikationen
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Durchflusszytometrie (FACS) (123), Western Blot (WB) (70), Immunpräzipitation (IP) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (23), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (14), Enzyme Immunoassay (EIA) (11), ELISA (5), ELISA (Detektionsantikörper) (3), Immunfluoreszenz (IF) (2), Funktionale Studien (Func) (1), Immunzytochemie (ICC) (1)
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Konjugate
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PE (20), FITC (17), Biotin (11), APC (5), Alexa Fluor 488 (5), DY647 (2), PE,Cy7 (2), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Dyomics 647 (1), Pacific Blue (1)
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Epitope
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AA 26-176 (4), Center E395 (1)
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