CD95 Antikörper (FITC)
| Antigen |
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| Klonalität |
Monoklonal (LT95) |
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Wirt
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Reaktivität
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Human (182), Maus (18), Ratte (Rattus) (9), Affe (7), Katze (3), Hund (3), Rind (Kuh) (1), Fruchtfliege (Drosophila melanogaster) (1), Hamster (1), Schwein (1), Kaninchen (1), Schaf (1), Xenopus laevis (1)
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Konjugat
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Applikation
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Durchflusszytometrie (FACS) (126), Western Blot (WB) (61), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (42), Immunpräzipitation (IP) (42), Enzyme Immunoassay (EIA) (31), Immunhistochemie (Paraffinschnitte) (IHC (p)) (26), Funktionale Studien (Func) (15), Immunfluoreszenz (IF) (12), Blockierende Antikörper (Inhibition) (8), ELISA (7), Apoptose-Induktion (AI) (4), Immunhistochemie (IHC) (4), Dot Blot (Dot) (3), ELISA (Detektionsantikörper) (3), Neutralisierung (Neut) (1)
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| Produktnummer |
ABIN238437 |
| Menge |
100 Tests |
| Preis |
230,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
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Weitere Bezeichnung
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CD95 / FAS
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Swiss-Prot
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P25445
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Immunogen
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HUT-78 human T cell lymphoma cell line
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Kreuzreaktivität
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Human
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Isotyp
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IgG1
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Klon
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LT95
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Beschreibung
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CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of theTNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular deathdomains, formation of the death-inducing signaling complex (DISC) and activation ofcaspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated atthe DISC, in type II cells low concentration of caspase 8 activates pathway leading to therelease of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokineresponses.
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Spezifität
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The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoproteinof the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily,expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although itcross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule. Species: Human. Others not tested.
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Applikationshinweise
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This antibody is suitable for Flow Cytometry analysis of human blood cells using 20 μlreagent/100 μl of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS containing 15 mM sodium azide as preservative and 0.2% (w/v)high-grade BSA (Protease free) as stabilizer.Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions.
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Lagerung
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Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf life: one year from despatch.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "CD95", Typ "Antikörper" finden
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Wirte
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Maus (154), Kaninchen (34), Ratte (6), Huhn (2), Ziege (1)
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Reaktivitäten
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Human (182), Maus (18), Ratte (Rattus) (9), Affe (7), Katze (3), Hund (3), Rind (Kuh) (1), Fruchtfliege (Drosophila melanogaster) (1), Hamster (1), Schwein (1), Kaninchen (1), Schaf (1), Xenopus laevis (1)
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Applikationen
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Durchflusszytometrie (FACS) (126), Western Blot (WB) (61), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (42), Immunpräzipitation (IP) (42), Enzyme Immunoassay (EIA) (31), Immunhistochemie (Paraffinschnitte) (IHC (p)) (26), Funktionale Studien (Func) (15), Immunfluoreszenz (IF) (12), Blockierende Antikörper (Inhibition) (8), ELISA (7), Apoptose-Induktion (AI) (4), Immunhistochemie (IHC) (4), Dot Blot (Dot) (3), ELISA (Detektionsantikörper) (3), Neutralisierung (Neut) (1)
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Konjugate
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FITC (20), PE (18), APC (8), Biotin (8), Alexa Fluor 488 (2), SPRD (2), Alexa Fluor 647 (1), PE,Cy5 (1)
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Epitope
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C-Term (4), pTyr232 (2), pTyr291 (2), Center (1)
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Alternativen 
