CHRNA3 Antikörper (AA 26-75)

Produktnummer ABIN6736366

Der Kaninchen Polyklonal anti-CHRNA3 Antikörper wird verwendet zum Nachweis von CHRNA3 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Pferd und Affe. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für CHRNA3 Antikörper (AA 26-75) (ABIN6736366)

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHRNA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CHRNA3 Antikörper

Bindungsspezifität: AA 26-75

Spezifität: Human CHRNA3

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit (100%) Bovine, Guinea pig (92%) Mouse, Rat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa26-75 of human CHRNA3 (P32297, NP_000734). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Ferret, Elephant, Dog, Bovine, Rabbit, Horse (100%), Pig, Opossum, Guinea pig (92%), Platypus (90%), Mouse, Rat, Hamster (85%), Turkey (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CHRNA3

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Andere Bezeichnung: CHRNA3

Hintergrund: Name/Gene ID: CHRNA3
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA3, AChR-alpha3, Acra-3, LNCR2, NAChR alpha 3, PAOD2, NACHRA3

Gen-ID: 1136

NCBI Accession: NP_000734

UniProt: P32297

Pathways: Synaptic Membrane