Band 3/AE1 Antikörper (AA 184-233)

Produktnummer ABIN6736430

Der Kaninchen Polyklonal anti-Band 3/AE1 Antikörper wird verwendet zum Nachweis von Band 3/AE1 in Proben von Human und Kaninchen. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für Band 3/AE1 Antikörper (AA 184-233) (ABIN6736430)

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Reaktivität: Human, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Band 3/AE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Band 3/AE1 Antikörper

Bindungsspezifität: AA 184-233

Spezifität: Human SLC4A1 / Band 3

Homologie: Percent identity by BLAST analysis: Human (100%) Rabbit (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa184-233 of human CHAMP1 (P02730, NP_000333). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Orangutan, Bat (92%), Marmoset (91%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Antibody has been tested in a peptide-based ELISA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Band 3/AE1

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Andere Bezeichnung: SLC4A1 / Band 3 / AE1

Hintergrund: Name/Gene ID: SLC4A1
Subfamily: Anion exchanger
Family: Transporter

Synonyms: SLC4A1, Anion exchanger 1, Anion exchanger-1, AE1, Anion exchange protein 1, Band 3, Band 3 anion transport protein, BND3, CD233, DI, EPB3, AE 1, Froese blood group, FR, RTA1A, SW, Swann blood group, WD1, WR, Wright blood group, WD, CD233 antigen, EMPB3, Waldner blood group

Gen-ID: 6521

NCBI Accession: NP_000333

UniProt: P02730