HSP90AB1 Antikörper (pSer254)

Produktnummer ABIN196935

Der Kaninchen Polyklonal anti-HSP90AB1 Antikörper wird verwendet zum Nachweis von HSP90AB1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für HSP90AB1 Antikörper (pSer254) (ABIN196935)

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSP90AB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-HSP90AB1 Antikörper

Bindungsspezifität: pSer254

Spezifität: The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. This antibody detects endogenous levels of HSP90B only when phosphorylated at Serine 254.

Aufreinigung: Immunoaffinity Chromatography using epitope-specific phosphopeptide.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-Sp-D-E).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu HSP90AB1

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Andere Bezeichnung: HSP90AB1 / HSP90 beta

Hintergrund: The 90- kDa heat shock protein (Hsp90) is a highly conserved, and abundant cytosolic homodimeric molecular chaperone. Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine and serine/threonine kinases and actin. Two isoforms which correspond to the major and minor isoform, (Hsp90 alpha and Hsp90 beta) can be found in nearly equal amount in humans, and operates as part of a multichaperone machinery in the cytosol, which includes Hsp70, peptidyl-prolyl isomerases and other cochaperones.Synonyms: HSP84, HSP90B, HSPC2, HSPCB, Heat shock 84 kDa, Heat shock protein HSP 90-beta

Gen-ID: 3326

NCBI Accession: NP_031381

UniProt: P08238

Pathways: Regulation of Cell Size