JunB Antikörper (pSer259)

Produktnummer ABIN196659

Der Kaninchen Polyklonal anti-JunB Antikörper wird verwendet zum Nachweis von JunB in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für JunB Antikörper (pSer259) (ABIN196659)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JunB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-JunB Antikörper

Bindungsspezifität: pSer259

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. JunB (phospho-Ser259) antibody detects endogenous levels of JunB only when phosphorylated at serine 259.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human JunB around the phosphorylation site of serine 259 (P-V-SP-P-I).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Andere Bezeichnung: Jun-B

Hintergrund: The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.Synonyms: JUNB, Transcription factor jun-B

Gen-ID: 3726

NCBI Accession: NP_002220

UniProt: P17275