CD53 (CD53) Antikörper (FITC)

Antigen: CD53 (CD53)

Synonyme: MOX44, TSPAN25

Klonalität: Monoklonal (MEM-53)

Wirt: Maus

Reaktivität: Human

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN192137

Produktbeschreibung

Weitere Bezeichnung: CD53

Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia.

Isotyp: IgG1

Klon: MEM-53

Beschreibung: CD53 is a tetraspanin family transmembrane glycoprotein expressed in thelymphoid-myeloid lineage. This molecule has been reported to form complexes with otherleukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37,CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved inradioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53is up-regulated in response to positive selection signals during T cell development, and isstrongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereasstimulation of neutrophils results in down-regulation of CD53 expression.

Spezifität: The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoproteinexclusivelly expressed on leukocytes, it is not present on platelets, red blood cells andnon-hematopoietic cells.The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of theantigen is reduced by 15 kDa using endoglycosidase F).HLDA IV, WS Code NL 59HLDA V, WS Code B CD53.5HLDA V, WS Code BP BP287HLDA V, WS Code T T-096HLDA V, WS Code X XB004

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar