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Nanog Homeobox Pseudogene 8 (NANOGP8) (C-Term) Antikörper

Antigen

Nanog Homeobox Pseudogene 8 (NANOGP8)

Synonyme ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog
Epitope
Alternativen

C-Term

Klonalität Polyklonal
Wirt
Alternativen

Ziege

Reaktivität
Alternativen

Human

Applikation
Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC), Enzyme Immunoassay (EIA)
1 Publikation vorhanden
Produktnummer ABIN190835
Menge 100µg  (100µg/200µl)
Preis 286,00 €   Zzgl. Versandkosten €40,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung NANOG
Gen-ID 79923
Swiss-Prot NP_079141.2
Immunogen Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.
Kreuzreaktivität Human
Format Liquid
Grade Serum-free
Beschreibung Official Symbol: NANOG
Spezifität Human

Anwendungen

Applikationshinweise Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1µg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources. IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Konzentration 100µg/200µl
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Lagerung Aliquot and store at -20°C. Minimize freezing and thawing.
Beschränkungen Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen Yasuda, Tsuneyoshi, Sumi et al.: "NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 9, pp. 1115-23, 2006 (PubMed).

Alternativen

Alternativen zu Antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", Typ "Antikörper" finden
Wirte Kaninchen (99), Maus (27), Ziege (10), Ratte (3)
Reaktivitäten Human (127), Maus (37), Ratte (Rattus) (26), Rind (Kuh) (5), Hund (5), Schwein (4), Huhn (3), Kaninchen (2), Zebrafisch (Brachydanio rerio) (2), Ziege (1), Pferd (1), Schaf (1)
Applikationen Western Blot (WB) (98), Immunfluoreszenz (IF) (53), Durchflusszytometrie (FACS) (42), ELISA (37), Enzyme Immunoassay (EIA) (29), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (8), Immunpräzipitation (IP) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunzytochemie (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blockierende Antikörper (Inhibition) (1), ELISA (Detektionsantikörper) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
Konjugate Biotin (7), Alexa Fluor 488 (3), Alexa Fluor 647 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitope C-Term (4), Center (3), N-Term (2), S285 (2), S71 (1)