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Antikörper
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anti-Nanog Homeobox Pseudogene 8 (NANOGP8) (C-Term) Antikörper
Nanog Homeobox Pseudogene 8 (NANOGP8) (C-Term) Antikörper
| Antigen |
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| Synonyme |
ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog |
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Epitope
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (127), Maus (37), Ratte (Rattus) (26), Rind (Kuh) (5), Hund (5), Schwein (4), Huhn (3), Kaninchen (2), Zebrafisch (Brachydanio rerio) (2), Ziege (1), Pferd (1), Schaf (1)
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Applikation
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Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC), Enzyme Immunoassay (EIA)
Western Blot (WB) (98), Immunfluoreszenz (IF) (53), Durchflusszytometrie (FACS) (42), ELISA (37), Enzyme Immunoassay (EIA) (29), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (8), Immunpräzipitation (IP) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunzytochemie (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blockierende Antikörper (Inhibition) (1), ELISA (Detektionsantikörper) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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| Produktnummer |
ABIN190835 |
| Menge |
100µg (100µg/200µl) |
| Preis |
286,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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NANOG
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Gen-ID
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79923
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Swiss-Prot
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NP_079141.2
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Immunogen
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Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.
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Kreuzreaktivität
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Human
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Format
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Liquid
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Grade
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Serum-free
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Beschreibung
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Official Symbol: NANOG
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Spezifität
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Human
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Applikationshinweise
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Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1µg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources. IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
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Konzentration
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100µg/200µl
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Reinigung
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Lagerung
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Yasuda, Tsuneyoshi, Sumi et al.: "NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 9, pp. 1115-23, 2006 (PubMed).
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Alternativen zu Antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", Typ "Antikörper" finden
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Wirte
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Kaninchen (99), Maus (27), Ziege (10), Ratte (3)
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Reaktivitäten
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Human (127), Maus (37), Ratte (Rattus) (26), Rind (Kuh) (5), Hund (5), Schwein (4), Huhn (3), Kaninchen (2), Zebrafisch (Brachydanio rerio) (2), Ziege (1), Pferd (1), Schaf (1)
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Applikationen
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Western Blot (WB) (98), Immunfluoreszenz (IF) (53), Durchflusszytometrie (FACS) (42), ELISA (37), Enzyme Immunoassay (EIA) (29), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (8), Immunpräzipitation (IP) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunzytochemie (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blockierende Antikörper (Inhibition) (1), ELISA (Detektionsantikörper) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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Konjugate
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Biotin (7), Alexa Fluor 488 (3), Alexa Fluor 647 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (4), Center (3), N-Term (2), S285 (2), S71 (1)
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