Dyskeratosis Congenita 1, Dyskerin (DKC1) Antikörper

Antigen: Dyskeratosis Congenita 1, Dyskerin (DKC1)

Synonyme: DKC, CBF5, NAP57, NOLA4, XAP101, FLJ97620, DKC1, fv62a07, MGC110395, wu:fa28f10, wu:fc87a02, wu:fi24a05, wu:fv62a07, zgc:110395, BC068171, MGC107501, RP23-62O13.1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN190831

Produktbeschreibung

Weitere Bezeichnung: DKC1

Gen-ID: 1736, 245474 (mouse)

Immunogen: C-KRKRESESESDETPP

Format: Affinity-purified

Beschreibung: All names and symbols: DKC1, dyskeratosis congenita 1, dyskerin , DKC, NAP57, NOLA4, XAP101,. Official Symbol: DKC1. RefSeq number(s): NP_001354.1

Spezifität: Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 70-75kDa band in Human Bone Marrow, Duodenum and Skin lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7kDa according to NP_001354.1. The 70-75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Spleen shows strong nuclear staining of select splenocytes. Recommended concentration, 5-10µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Chromatin-Bindungsproteine, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN190831 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publikationen

Publikationen: Yoon, Peng, Brandenburger et al.: "Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita." in: Science (New York, N.Y.), Vol. 312, Issue 5775, pp. 902-6, 2006 (PubMed).