ATP6V0D2 Antikörper (epsilon subunit)

Produktnummer ABIN3197488

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-ATP6V0D2 Antikörper wird verwendet zum Nachweis von ATP6V0D2 in Proben von Arabidopsis thaliana, Nicotiana tabacum, Tomato, Oryza sativa, Alfalfa, Mais (Zea mays), Vitis vinifera, Apple, Thellungiella, Gerste, Chara australis, Chlamydomonas reinhardtii, Cucumis sativus, Fortunella margarita, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Noccaea caerulescens, Petunia hybrida, Pineapple, Poplar (Populus), Pteris vittata (Chinese ladder brake), Salicornia bigelovii und Triticum aestivum. Er wurde validiert für WB, IF und IHC.

Kurzübersicht für ATP6V0D2 Antikörper (epsilon subunit) (ABIN3197488)

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Reaktivität: Arabidopsis thaliana, Nicotiana tabacum, Tomato, Oryza sativa, Alfalfa, Mais (Zea mays), Vitis vinifera, Apple, Thellungiella, Gerste, Chara australis, Chlamydomonas reinhardtii, Cucumis sativus, Fortunella margarita , Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Noccaea caerulescens, Petunia hybrida, Pineapple, Poplar (Populus), Pteris vittata (Chinese ladder brake), Salicornia bigelovii, Triticum aestivum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6V0D2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-ATP6V0D2 Antikörper

Bindungsspezifität: epsilon subunit

Verwendungszweck: Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase

Kreuzreaktivität (Details): Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Salicornia bigelovii, Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in: Avicennia sp., mangrove plants, Schizosaccharomyces pombe

Homologie: Brachypodium dystachyon,Capsella rubella,Chenopodium quinoa,Citrus clementina,Citrus unshiu,Citrus limon,Eucalypsus grandis,Glyxine max,Glycine soja,Lotus japonicus,Phaseolus sp.,Physcomitrium patens,Populus trichocarpa,Prunus persica,Ricinus communis,Riticum aestivum,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor Theobroma cacao,Vitis vinifera,Bull frog,Chicken,Bovine,Drosophila melanogaster,Human,Mouse,Rat

Aufreinigung: Serum

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1

Anwendungsinformationen

Applikationshinweise: 1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)

Kommentare:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-ATP6V0D2 Antikörper (ABIN3197488)

Bigeard, Rayapuram, Bonhomme, Hirt, Pflieger: "Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." in: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).

Schnell, Han, Miki, Johnson: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." in: Plant cell reports, (2010) (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).

Details zu ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Andere Bezeichnung: V-ATPase

Hintergrund:

Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Additional information: Cellular [compartment marker] of tonoplast membrane.This product can be sold containing ProClin if required.

Molekulargewicht: 26 | 31 kDa (Arabidopsis thaliana)

Gen-ID: 826716

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport