Epsilon subunit of tonoplast H+ATPase (V-ATPase ) Antikörper

Antigen: Epsilon subunit of tonoplast H+ATPase (V-ATPase )

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Pineapple (Ananas comosus), Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii), Barley (Hordeum vulgare), Tomate (Solanum lycopersicum), Easter lily (Lilium longiflorum), Ficoidaceae (Mesembryanthemum), Tabak (Nicotiana tabacum), Petunia, Farne, Thellungiella, Maize/Corn (Zea mays)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN190733

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPaseincluding Arabidopsis thaliana At4g11150. Peptide is conserved in valuolarH+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Format: Lyophilized

Beschreibung: Plant vacuole V-ATPase is responsible for energization of transport of ions andmetabolites, and acts as well 'house-keeping' and as a stress response enzyme.V-ATPase is a multi-subunit enzyme composed of a membrane sector and acytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternativeprotein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE2448

Spezifität: Predicted reactivity: dicots including Phaseolus sp. , Ricinus communis, Vitis vinifera and monocotsincluding: Oryza sativa, Zea mays, algae, Physcomitrella patens, bull frog, chicken, bovine, Drosophila melanogaster,human, mouse, rat.

Anwendungen

Applikationshinweise: Recommended Dilution 1 : 2 000 - 1: 5000 with alkaline phosphatase (WB), 1:50 (IHC).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot analysis of samples from yeast whole cell extract expressing the Arabidopsis thaliana subunit E1. Primary antibodies have been used at 1: 1: 000. Secondary antibodies at 1: 3 000. The load per well was 10 ug of total protein. Samples were separated on 10 % gel in Tris glycine SDS buffer. The proteins were transferred to a membrane (Schleicher & Schuell), blocked with 5% dry milk in Tris-buffered saline before incubation for 2 hours up to over night at RT with the primary antibody. A series of washes with TTBS was followed by incubation with a secondary antibody from).

Western Blotting: 1 - molecular weight markers (Precision Plus Protein Standards, Dual Color, Bio-Rad, 10ul), 2 -Arabidopsis thaliana leaf extract (25 ug) Experimental Conditions: Protein was separated on a 12% SDS-PAGE gel at 200 V for approximately 40 min using the mini-Protein 3 cell (Bio-Rad). Protein was transferred to PVDF at 100 V for 1h using the mini-TransBlot cell (Bio-Rad). Blocked overnight at 4ºC in 5% non-fat milk dissolved in 1xPBST. Incubated with anti-V-ATPase at 1:2 000 for 2h at RT. After several washes in 1xPBST, blot was incubated with goat anti-rabbit IgG-alkaline phosphatase conjugate (Bio-Rad) at 1:3000 for 1h at RT. Blot was developed with NBT/BCIP (Molecular Probes). Courtesy: Ms Jaimie Schnell, PhD candidate

Publikationen

Publikationen: Hinton, Sennoune, Bond et al.: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, 2009 (PubMed).

Schnell, Han, Miki et al.: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." in: Plant cell reports, 2010 (PubMed).