Nonhomologous End-Joining Factor 1 (NHEJ1) Antikörper

Antigen: Nonhomologous End-Joining Factor 1 (NHEJ1)

Synonyme: XLF, FLJ12610, MGC130191, MGC130192, cernunnos, 1700029B21Rik, NHEJ1, MGC139115, nhej1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN185719

Produktbeschreibung

Weitere Bezeichnung: XRCC4-like factor / NHEJ1

Gen-ID: 79840

Swiss-Prot: NP_079058.1

Immunogen: Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.

Kreuzreaktivität: Human, Hund

Format: Liquid

Grade: Serum-free

Beschreibung: Official Symbol: NHEJ1

Spezifität: Human, Dog 299 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3µg/ml. IHC: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8µg/ml.

Konzentration: 100µg/200µl

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185719 (0.1 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Ahnesorg, Smith, Jackson: "XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining." in: Cell, Vol. 124, Issue 2, pp. 301-13, 2006 (PubMed).