Serotonin Receptor 3A (HTR3A) Antikörper

Antigen: Serotonin Receptor 3A (HTR3A)

Synonyme: HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R, 5-HT3, 5HT3, Htr3, MGC52789, HTR3A, Htr3a

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185673

Produktbeschreibung

Weitere Bezeichnung: Serotonin receptor 3A / HTR3A

Gen-ID: 3359

Immunogen: C-GPQDFEKSPRDR

Format: Affinity-purified

Beschreibung: All names and symbols: HTR3A, serotonin receptor, 5-hydroxytryptamine (serotonin) receptor 3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine (serotonin) receptor-3, 5HT3 serotonin receptor, Serotonin-gated ion channel receptor, truncated receptor, containing only 3 transmembrane domains. Official Symbol: HTR3A. RefSeq number(s): NP_998786.1, NP_000860.1

Spezifität: This antibody is expected to recognise both reported isoforms (NP_998786.1 and NP_000860.1). Human 510 aa, 478 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.1. Recommended concentration: 0.3-1µg/ml. A minor band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185673 (0.3µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Dremencov, Weizmann, Kinor et al.: "Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response." in: Current drug targets, Vol. 7, Issue 2, pp. 165-75, 2006 (PubMed).