CYP17A1 Antikörper (Internal Region)

Produktnummer ABIN185617

Dieser Ziege Polyklonal Antikörper detektiert spezifisch CYP17A1 in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CYP17A1 Antikörper (Internal Region) (ABIN185617)

Target: CYP17A1 (Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 (CYP17A1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CYP17A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-CYP17A1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CYP17A1

Sequenz: LFKDGDQKLE K

Kreuzreaktivität: Cat, Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LFKDGDQKLEK, from the internal region of the protein sequence according to NP_000093.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Ovary lysates (calculated MW of 57.4 kDa according to NP_000093.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CYP17A1

Target: CYP17A1 (Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 (CYP17A1))

Andere Bezeichnung: CYP17A1

Hintergrund: CYP17A1, cytochrome P450, family 17, subfamily A, polypeptide 1 , HGNC:2593, CPT7, CYP17, P450C17, S17AH, cytochrome P450, family 17, cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia, cytochrome p450 XVIIA1, steroid 17-a

Gen-ID: 1586

NCBI Accession: NP_000093

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin