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Antikörper
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anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) Antikörper
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antikörper
| Antigen |
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| Synonyme |
FLJ46390, TCF1ALPHA, DKFZp586H0919, LEF-1, LEF1 |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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Western Blot (WB) (68), Immunfluoreszenz (IF) (31), Durchflusszytometrie (FACS) (20), Enzyme Immunoassay (EIA) (12), ELISA (10), Immunhistochemie (Paraffinschnitte) (IHC (p)) (8), Immunhistochemie (IHC) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), ELISA (Detektionsantikörper) (1), Gel Shift (GS) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN185607 |
| Menge |
100 ug (Varianten) |
| Preis |
286,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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LEF1
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Gen-ID
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51176, 16842 (mouse), 161452 (rat)
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Immunogen
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C-QHEQRKEQEPKRPH
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Format
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Affinity-purified
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Beschreibung
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All names and symbols: LEF1, lymphoid enhancer-binding factor 1 , HGNC:6551, DKFZp586H0919, TCF1ALPHA , lymphoid enhancer binding factor-1. Official Symbol: LEF1. RefSeq number(s): NP_057353.1
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Spezifität
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Human, Mouse, Rat, Dog 399 aa
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Applikationshinweise
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Peptide ELISA: antibody detection limit dilution 1:32,000. Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3µg/ml.
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Reinigung
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Lagerung
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Forschungsgebiet
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Transkriptionsfaktoren, Wnt, Abstammungsmarker
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004 (PubMed).
Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005 (PubMed).
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Alternativen zu Antigen "Lymphoid Enhancer-Binding Factor 1 (LEF1)", Typ "Antikörper" finden
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Wirte
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Maus (44), Kaninchen (31), Ziege (7), Schaf (5)
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Reaktivitäten
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Human (76), Maus (33), Ratte (Rattus) (26), Hund (22), Rind (Kuh) (20), Schwein (19), Kaninchen (18), Huhn (16), Pferd (1)
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Applikationen
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Western Blot (WB) (68), Immunfluoreszenz (IF) (31), Durchflusszytometrie (FACS) (20), Enzyme Immunoassay (EIA) (12), ELISA (10), Immunhistochemie (Paraffinschnitte) (IHC (p)) (8), Immunhistochemie (IHC) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), ELISA (Detektionsantikörper) (1), Gel Shift (GS) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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HMG Binding Domain (2), all isoforms (1), alternate exon (1), transactivation domain (1)
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Alternativen 
